1.
Indian J Biochem Biophys
;
1994 Aug; 31(4): 339-43
Article
in English
| IMSEAR
| ID: sea-26653
ABSTRACT
DNA topoisomerase I has been purified from Mycobacterium smegmatis to near homogeneity using different column chromatographic techniques. The enzyme activity relaxes form I DNA into form IV DNA, requiring Mg2+, but not ATP or any other cofactors for its activity. Several properties of the enzyme were found to be similar to that of the prototype enzyme, Escherichia coli topoisomerase I.